Simple calibration:
The operations to perform for a calibration are:
1) Copy your peak list to the clipboard from your favourite spectrum analysis program like m/z or Protein Explorer.
2) Paste the list into PeakErazor using the “Read from clip.” button. Make sure the graph is displayed to guide you through the calibration.
3) Adjust your settings: Pressing the arrow to the left of the Precision field will set the field to the maximum possible (e.g. 800 ppm) - good for locating an off-center calibration line; the right-hand arrow will switch to a detailed view with high precision (e.g. 40 ppm). In the ‘Remove from list’ box you can check/uncheck the ‘Erazor list’ fields you want to remove. Individual m/z values can be unchecked from the peak list itself.
4) Click on the ‘Calibrate’ button to go the calibration page.
5) Perform multipoint calibration (see below).
6) Switch back to the main window by click on ‘Calib done’. This will switch you back to input tab.
7) Copy the calibrated peak list back onto the clipboard by pressing ‘Copy to clip.’.
6-7a) As an alternative to go through the input tab of the program, you can press the ‘Done and copy’ button which will do the point 6) and 7) in a single operation (i.e. copy the calibrated list to the clipboard and switch to the input tab).
8) Paste the list into your favourite peptide mapping search program (e.g. Mascot, ProFound etc.).
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